Et Terminator Kit for Automated Dna Sequencing Were from Amersham Pharmacia Biotech. Sweden. X-ray Films and Intensifying Screens Were Purchased from Kodak, Usa. Dna Restriction and Modifying Enzymes, Dna and Protein Molecular Weight Markers and Rnase Were Purchased from New

Agarose, EDTA. calf-thymus DNA, ethidium bromide, glucose, (3mercaptoethanol, SDS, sodium acetate. NaCI, PEG, Tris, PVP, formamide, formaldehyde, guanidine isothiocyanate. glycerol, ABA were obtained from Sigma chemicals, USA. The streptavidin linked paramagnetic beads were purchased from Novagen. USA. Chemicals for bacterial media preparation and solvents phenol, ethanol, glacial acetic acid, acetone, chloroform, methanol. and DMSO of analytical grade, purchased from local sources. Oligotex suspension was purchased from Qiagen GmbH, Germany. The DNA labeling kits were obtained from Life Technologies, Rockville, Md. or BARC. India. Hybond N+ for nucleic acid transfer and DYEnamic ET terminator kit for automated DNA sequencing were from Amersham Pharmacia Biotech. Sweden. X-ray films and intensifying screens were purchased from Kodak, USA. DNA restriction and modifying enzymes, DNA and protein molecular weight markers and RNAse were purchased from New England Biolabs, Beverly, Mass. USA, Bangalore Genei Pvt. Ltd., Bangalore and MBI Fermentas, Lithuvania. NickelCL agarose was obtained from Bangalore Genei Pvt. Ltd.. India.